The basics - continued 3

DNA and RNA

The hereditary information of an organism is stored in the DNA in the form of a code. This code consists of four building blocks or bases (A for Adenine, C for Cytosine, G for Guanine, T for Thymine). These bases follow each other in a certain sequence, e.g. ...AGTCGTAATTGGCCCCAATTGCAAAAA.... If such a sequence contains the information for a single protein, it is called a gene.

The DNA-backbone consists of sugars (deoxyribose) and phosphates. Each sugar has a base bonded to it: either adenine, guanine, cytosine or thymine. DNA occurs as a double helix with the two chain held together by hydrogen bonds between the bases, the so-called  base pairs. Adenine is always paired with thymine, and guanine with cytosine.

Exercise 14:

Fill in the following names in the figure below: adenine, thymine, cytosine, guanine, uracil, phosphate, deoxyribose, hydrogen bond, backbone, and bases. Answer

Exercise 14

Exercise 15:

A piece of  DNA contains 33% guanine. What are the percentages of adenine, cytosine, and thymine in that piece of DNA? Answer

In RNA deoxiribose is replaced by ribose and thymine by the base uracil. RNA is usually found as a single chain. However, this chain can fold by making hydrogen bonds (base pairs) with itself, thus forming a typical 3D structure.

Exercise 16:

Name two types of RNA that are known for their typical structure. Answer

Transcription and translation

Transcriptie en transalatieThe DNA sequence forms the basis for proteins. Enzymes make an RNA-copy of one of the chains in the double helix. This messenger RNA (mRNA) contains the code that dictates the amino acid sequence of the protein product. The actual synthesis of proteins is done on the ribosomes.

The creation of mRNA is called transcription. The creation of proteins using the information in mRNA is called translation. Below is a schematic overview.

Transcription and transalation

It is important to realise that a single piece of DNA, one gene, will code for a distinct protein.

You can look up the RNA code in a codon table: you can figure out which amino acid is encoded in a triplet of bases (i.e. a codon).

Exercise 17:

A piece of (single strand) DNA has the following sequence:

TACCATCCGTTCATGATC

For which peptide does it code? Answer

If you understood all the questions, you should have a firm grasp of the basics needed to use this site. You can now continue to a short introduction in the field of bioinformatics.

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